K-562-Cas9

Price : $1980 $2480
Catalog No. : VAS3B002
Size : 1*10^6
Lead time : 2-3 weeks

This K-562 Cas9 stable cell line is made by lentivirus. Cas9 is stably expressed, and the Cas9 activity is validated by gene editing experiments. It is available in stock, and the lead time is 2-3 weeks. Please feel free to contact us for more cell in

Product name
K-562-Cas9
Catalog No.
VAS3B002
Expressed protein
Cas9 Nuclease
Genotype
Multi-clonal
Selection marker
Puromycin,GFP
Host cell line
Human myelogenous leukemia cell line (K-562)
Adherent /Suspension
Suspension
Morphology
Lymphocyte-like
Biosafety level
2
STR profiling
D5S818: 11,12; D13S317: 8,8; D7S820: 9,11; D16S539: 11,12; vWA: 16,16; THO1: 9.3,9.3; Amelogenin: X,X; TPOX: 8,9; CSF1PO: 9,10
Application
Gene editing (knockout, point mutation and tag knockin)
CRISPR library screening
Complete culture media
90%IMDM+10%FBS
Freezing media
50%IMDM+40%FBS+10%DMSO
Special note
Please thaw the cells in T25. Please do not thaw the cells directly to a T75 flask or 10 cm culture dish.
Receiving and storage instructions
1.Upon arrival, check all containers for leakage or breakage.
2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. Please do not store at -80°C. Storage at -80°C may result in loss of viability.
Initial Handling procedure
1.Pipette 6-7 mL of complete medium into a 15 mL centrifuge tube.
2.Gently agitate the vial in a 37°C water bath to thaw. Please keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1-2 minutes).
3.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol or wiping with an alcohol cotton pellet. All of the operations from this point on should be carried out in the hood and under strict aseptic conditions.
4.Transfer the vial contents to the step 1 centrifuge tube containing 6-7 mL complete culture medium. And spin at 1100 rpm for 4 mins at room temp to collect the cells.
5.Resuspend cell pellet with 1ml recommended complete medium. And dispense into a T25 flask (or 6 cm culture dish) containing 4 mL of complete medium. (Note: It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH 7.0 to 7.6.)
6.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
Subculturing procedure
1.Addition of fresh medium: gently pipette the cells in the flask and take 20 ul of cells for cell counting; According to the cell counting results, aspirate and discard part of the cell suspension, adjust the cell density to 2x10^5~4.0x10^5 cells/mL.
2.Replace fresh medium when a lot cell debris and the medium turned yellow. Centrifuge at 1100 rpm for 4 minutes to collect cells; Resuspend cells and adjust the cell density to 2x10^5~4.0x10^5 cells/mL.

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Vitro Biotech offers cell engineering products and services about KO cells, Luciferase cells, Cas9 expressing cell lines, and various stable cell lines.