AAV packaging
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As gene therapy continues to revolutionize modern medicine, Adeno-Associated Virus (AAV) has emerged as one of the most promising tools for delivering genetic material into cells. AAV has become the go-to platform for gene therapy due to its non-pathogenic nature, high specificity, high transduction efficiency, and stability in vivo. AAV is an incredibly versatile and powerful tool for gene therapy applications. Its high transduction efficiency, ability to target specific cells or tissues, and long-term expression make it an attractive platform for gene therapy treatments. With ongoing research and development, AAV-based gene therapy has the potential to transform modern medicine and provide new treatments for a wide range of diseases.
▍ Vitro Biotech Offers An Exceptional AAV Packaging Service
We provide high titer AAV for in vitro transduction, and ultra-purified AAV for in vivo study. Feel free to contact us to get a quote >>
At its core, AAV packaging is the process of producing virus particles that carry a specific genetic payload. Here are what our AAV packaging service include:
✔ Overexpression AAV: Constitutively express gene of interest.
✔ Knockdown AAV: Carrying a shRNA that can be used to suppress gene expression.
✔ CRISPR/Cas9 AAV: Carrying components for targeted gene editing. Useful tool for gene knockin experiment.
✔ Cre AAV: Useful for studies with floxed mice.
▍ Quality control measures
1) Sanger sequencing to verify the correct sequence is carried.
2) Cell transduction test and RT-qPCR to determine AAV titer.
3) Mycoplasma test to ensure contamination free!