Having trouble generating the CRISPR KO cell line? Perhaps your gene is essential for the cell line. Why not try to generate an shRNA knockdown stable cell line instead?

▍  shRNA-mediated Gene Knockdown

The shRNA knockdown stable cell line project generally uses lentivirus to introduce an shRNA into the target cell genome to achieve the long-term stable expression of shRNA in the cell line. So as to induce the RNA interference effect, which could diminish the expression level of the target gene. Unlike siRNA transient transfection, shRNA stable cell line has a long-term expression of shRNA, and has a more stable gene knockdown effect.

▍  Features

With many years of experience in cell biology, Vitro Biotech provides technical services for the construction of lentivirus shRNA knockdown stable cell lines. Feel free to contact us to get a strategy for your lentiviral knockdown cell line project.

Knockdown cell line workflow