CRISPR Library Screening

CRISPR library screening is a powerful tool to study gene function and identify target genes, which greatly simplifies the screening of functional genes. In addition to the most commonly used CRISPR gene knockout sgRNA library, there are also transcriptional activation (CRISPRa) library and CRISPR interference (CRISPRi) library. Vitro Biotech provides a complete solution for CRISPR library screening. Welcome to contact us to get an exclusive strategy.

▍  CRISPR Library Classification

The CRISPR whole genome sgRNA library can knockout any human or mouse genes and non-coding genes, such as miRNA genes. Each sgRNA is cloned into the optimized lentiviral vector to achieve efficient transduction of primary cells or cell lines. The cells should be transduced by pooled lentiviral library with low MOI to ensure that no more than 1 sgRNA gets in each cell. After transduction and before screening, NGS should be performed to evaluate the sgRNAs performance in cells. After screening, another round of NGS and data analysis is needed to identify the sgRNA that is missing or enriched during the screening process.

Sub-pool library targets at genes involved in some specific pathways or biological processes. Customized sgRNA library for CRISPR screening is an important means in functional genomics research. Unlike the whole genome CRISPR library, the customized library can be optimized for the selected cell type, further improving the efficiency and feasibility of screening.

▍  Vitro Biotech’s popular libraries

Type	Species	Gene coverage	Vector system
Knockout library	Human	Whole genome	Lentivial one or two plasmids system
Knockout library	Mouse	Whole genome	Lentivial one or two plasmids system
CRISPRa library	Human	Whole genome	Lentivial two or three plasmids system
CRISPRa library	Mouse	Whole genome	Lentivial two or three plasmids system
Knockout library	Human	1880 unknown function genes	Lentivial two plasmids system
Knockout library	Human	507 kinase genes	Lentivial two plasmids system
Customized sgRNA library	Upon request		Lentivial one, two or three plasmids system

For a complete portfolio of Vitro Biotech's CRISPR libraries, please visit here.

▍  Vitro Biotech Complete Solution for Gene Function Screening

Typically, procedures of CRISPR gene screening can be summarized into three aspects: sgRNA library development, functional screening and target validation. The preliminary design and strategy of each step will affect the final screening results. Relying on rich experience in gene editing, Vitro Biotech can provide a complete solution for CRISPR library screening. Whether it's library customization, lentivirus library production, or cell screening, Vitro Biotech can help you to accomplish this with affordable prices and professional service!

Gene Function Screening steps:

Vitro Biotech offers the following phenotype CRISPR library screenings:

▲ Screening of tumor metastasis related genes (in vivo)
▲ Screening of cell migration/invasion related genes
▲ Screening of cell colony formation related genes
▲ Screening of cell proliferation related genes
▲ Screening of tumor drug resistance related genes
▲ ......

The CRISPR library screening service provided by Vitro Biotech can help you further simplify the gene screening process, improve the screening efficiency, and assist your innovative research!