Lentivirus packaging
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Lentivirus packaging is a powerful tool for researchers working in the field of genetic engineering and gene therapy. Stable cell line generation lentivirus allows for efficient delivery of genetic material to a wide range of cell types, including difficult-to-transfect cells like neurons or immune cells (eg. THP-1), primary cells and stem cells. This makes them an excellent choice for both basic research and clinical applications.
▍ Vitro Biotech Offers An Exceptional Lentivirus Packaging Service
We provide high titer lentivirus for in vitro transduction, and ultra-purified lentivirus for in vivo study. So why not consider incorporating lentivirus into your next experiment or project? Get a quote now>>
At its core, lentivirus packaging is the process of producing lentiviral particles that carry a specific genetic payload. Here are what our lentivirus packaging service include:
✔ Overexpression lentivirus: Constitutively express gene of interest.
✔ Knockdown lentivirus: Carrying a shRNA that can be used to suppress gene expression.
✔ CRISPR/Cas9 lentivirus: Carrying components for targeted gene editing.
✔ Inducible expression lentivirus: Express the introduced gene under specific conditions, eg. Doxycycline induced Tet-on system.
▍ Quality Control Measures
1) Sanger sequencing to verify the correct sequence is carried.
2) Cell transduction test and RT-qPCR to determine lentivirus titer.
3) Mycoplasma test to ensure contamination free!
▍ Lentivirus-meidated Stable Cell Line Generation
Lentivirus is a valuable tool for genetic engineering applications due to its versatility, efficiency, and specificity. If you are looking to introduce a new gene to cells, study gene function, or perform targeted gene editing, consider exploring the potential of lentivirus packaging for your research!
Stable cell line generation lentiviruses integrate their DNA into the host cell genome, which means that the introduced genes will be passed on to daughter cells as well. This makes lentivirus-mediated gene transfer particularly useful for applications where long-term expression is desired, such as for the generation of stable cell lines. Vitro Biotech also offer stable cell line customization service, 100+ types of cell lines are feasible, as soon as 6 weeks!