--CRISPR/Cas9 Gene Editing Technology

Introduction of CRISPR/Cas9-mediated genome editing techniques has revolutionized the field of molecular biology, offering precise and efficient methods for manipulating genes in various organisms.


▍  CRISPR/Cas9 Knockout

At present, there is an ever-increasing demand for such KO cell lines as they provide an excellent research tool in the fields of genomics and medicine. Researchers can use these cells to study the role of a specific gene in normal cellular processes or in the context of a disease model. They can also be used to validate drug targets, screen potential therapeutic compounds, and aid in drug development.


▍  CRISPR/Cas9 Knockin

CRISPR gene knock-in is mainly divided into two directions, one is point mutation introduction, and the other is tag knock in to the target gene. Point mutation cell line is a very useful means in cell gene research methods, which can study the function of point mutation in situ in cells. Tag knock in cell line can locate and quantify the target protein.


▍  CRISPR/Cas9 Gene Activation

CRISPRa (Transcriptional Activation) system is a powerful tool for activating the transcription of endogenous genes. Mutated Cas9 (dCas9) connects with the transcriptional activator (such as VP64, p65 and HSF1) can effectively promote the transcription of specific genes. The gene transcription can be increased by two to thousands folds.

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