THP-1 LUC-GFP
Price :
$2000
$2800
Catalog No. :
VLG2B024
Size :
1*10^6
Lead time :
2-3 weeks
This THP-1 luciferase and EGFP co-expression cell line is made by lentivirus. Luciferase and EGFP are stably expressed, and the luciferase activity is validated. Please feel free to contact us for more cell information and order placing.
Product name
THP-1-LUC-EGFP
Catalog No.
VLG2B024
Expressed protein
Luciferase, EGFP
Genotype
Multi-clonal
Selection marker
Puromycin
Host cell line
THP-1
Adherent /Suspension
Suspension
Morphology
Please inquire
Biosafety level
1
Application
Efficacy evaluationIn vivo imaging
In vitro imaging
Vitro Biotech offers cell engineering products and services about KO cells, Luciferase cells, Cas9 expressing cell lines, and various stable cell lines.
2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. Please do not store at -80°C. Storage at -80°C may result in loss of viability.
2.Gently agitate the vial in a 37°C water bath to thaw. Please keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1-2 minutes).
3.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol or wiping with an alcohol cotton pellet. All of the operations from this point on should be carried out in the hood and under strict aseptic conditions.
4.Transfer the vial contents to the step 1 centrifuge tube containing 6-7 mL complete culture medium. And spin at 1100 rpm for 4 mins at room temp to collect the cells.
5.Resuspend cell pellet with 1ml recommended complete medium. And dispense into a T25 flask (or 6 cm culture dish) containing 4 mL of complete medium. (Note: It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH 7.0 to 7.6.)
6.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.