LN-229-Luc
Price :
$1400
$1700
Catalog No. :
VLU5B479
Size :
1*10^6
Lead time :
2-3 weeks
This SBC-5 luciferase stable cell line is made by lentivirus. Luciferase is stably expressed, and the luciferase activity is validated. Please feel free to contact us for more cell information and order placing.
Product name
LN-229-Luc
Catalog No.
VLU5B479
Expressed protein
Luciferase
Genotype
Multi-clonal
Selection marker
Puromycin
Host cell line
Human glioma cell line (LN-229)
Adherent /Suspension
Adherent
Morphology
Epithelial-like
Biosafety level
2
Application
Efficacy evaluationIn vivo imaging
Vitro Biotech offers cell engineering products and services about KO cells, Luciferase cells, Cas9 expressing cell lines, and various stable cell lines.
2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. Please do not store at -80°C. Storage at -80°C may result in loss of viability.
2.Immediately spin at 1000 rpm for 3 mins at room temp to collect the cells.
3.Discard freezing media. Resuspend cell pellet with 1ml recommended complete medium. And dispense into a T25 flask (or 6 cm culture dish) containing 4 mL of complete medium. (Note: It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH 7.0 to 7.6.)
4.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
2.Rinse the cells 1-2 times with 1xPBS (2-3mL for T25 flask, increase or decrease the amount needed proportionally for culture vessels of other sizes) to remove residual medium and serum.
3.Add 1 mL of Trypsin-EDTA solution to T25 flask (increase or decrease the amount needed proportionally for culture vessels of other sizes) allow trypsin completely cover the cells, then place the flask into the incubator and incubate for 1-2 mins (if cells are hard to detach, allow appropriate extension of incubation), and observe cells under an inverted microscope until cell layer is dispersed. (Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.)
4.Add 2 times of trypsin vloume of complete growth medium to stop digestion, and aspirate cells by gently pipetting.
5.Transfer the cell suspension with a 10 mL pipette into a 50 mL centrifuge tube, rinse the residual cells from the flask with PBS , then collect to the centrifuge tube.
6.Centrifuge at 1000 rpm for 5 mins at room temp. Then remove and discard the supernatant and resuspend the cells with 2 mL of complete medium.
7.Dispense the cells into a suitable culture vessel containing corresponding amount of complete medium.
8.Incubate cultures at 37°C.