Luciferase Reagents

Luciferase is a general term for a class of oxidative enzymes used for bioluminescence, the most commonly used luciferase are firefly luciferase (F-Luc) (Photinus pyralis), Renilla luciferase (R-Luc), Nano-luciferase(N-Luc), Luc1, and Luc2.

For different kinds of luciferase, Vitro Biotech provides various types of substrates and assay kits, such as Luc2 substrate, Luc1 substrate, CycLuc 1, D-Luciferin sodium, D-Luciferin, Coelenterazine, luciferase assay kit, and dual luciferase assay kit.

Properties of Different Luciferase Enzymes:

Types
Wavelength Peak
Substrate
Require ATP
Sensitivity
F-Luc
565 nm
D-Luciferin
YES
Medium
R-Luc
480 nm
Coelenterazine
NO
Medium
N-Luc
460 nm
Coelenterazine
NO
High
Luc1
547 nm
Luc1 Substrate (DTZ Solution)
NO
High
Luc2
547 nm
Luc2 Substrate (AkaLumine-HCl)
NO
High


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Vitro Biotech offers cell engineering products and services about KO cells, Luciferase cells, Cas9 expressing cell lines, and various stable cell lines.