Luciferase is a general term for a class of oxidative enzymes used for bioluminescence, the most commonly used luciferase are firefly luciferase (F-Luc) (Photinus pyralis), Renilla luciferase (R-Luc), Nano-luciferase(N-Luc), Luc1, and Luc2.
For different kinds of luciferase, Vitro Biotech provides various types of substrates and assay kits, such as Luc2 substrate, Luc1 substrate, CycLuc 1, D-Luciferin sodium, D-Luciferin, Coelenterazine, luciferase assay kit, and dual luciferase assay kit.
Properties of Different Luciferase Enzymes:
Types |
Wavelength Peak |
Substrate |
Require ATP |
Sensitivity |
F-Luc |
565 nm |
D-Luciferin |
YES |
Medium |
R-Luc |
480 nm |
Coelenterazine |
NO |
Medium |
N-Luc |
460 nm |
Coelenterazine |
NO |
High |
Luc1 |
547 nm |
Luc1 Substrate (DTZ Solution) |
NO |
High |
Luc2 |
547 nm |
Luc2 Substrate (AkaLumine-HCl) |
NO |
High |