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Luciferase Stable Cell Line Application: In Vivo Imaging
The luciferase stable cell line was then injected to mice. A single injection of luciferin (the substrate of luciferase) into model mice can maintain the luminescence of luciferase labeled cells in their bodies for 30-45 minutes....
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Luciferase Reporter Cell Line Application: New Drug/Treatment
Luciferase reporter cell lines are widely used in drug discovery. The following are three common in vitro applications of luciferase reporter cell line in drug/therapeutic research:...
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Everything You Need To Know About CRISPR gRNA Library Screeni
CRISPR screen library is an important tool to identify which genes are important for the target phenotype. In this article, we will introduce how to use the CRISPR gRNA library and CRISPR Cas9 cell line for genome wide screening....
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Weak Spot of Lung Cancer Protein Uncovered
Rendering of interface in the mutant EGFR studied that was shown to be critical for EGFR-driven tumour growth. The K946E mutation shown in red was used by the team to confirm that this interface is implicated in tumour growth in the study....
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Brake Protein Could Give the Green Light to Novel Cancer Ther
Now, researchers at Stanford University and New York University (NYU) have discovered new insights into one of these brake proteins, LAG-3....
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Luciferase in vivo imaging protocol
This manual introduces how to perform mouse in vivo imaging of luciferasse....
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Protocol: CRISPR gene knockout protocol (Part 3): Single Cell
Among the CRISPR gene knockout protocols, the most commonly used and efficient method for CRISPR KO cell line generation is to use gRNA vectors to transfect into Cas9 stable cell lines...
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Protocol: CRISPR-Cas9 knockout protocol (Part 2): Cell Transf
Vitro Biotech prepared a crispr-cas9 knockout protocol to guide you step by step to generate the crispr/cas9 KO cell line.Here is the part 2 of the protocol--Cell Transfection....